Effect of chitosan on epithelial permeability and structure
Effect of chitosan on epithelial permeability and structure Effect of chitosan on epithelial permeability and structure Dodane, Valrie; Amin Khan, M.; Merwin, June R. 1999-05-10 00:00:00 Numerous studies have shown that chitosan, a mucoadhesive polymer, is a potential enhancer for transmucosal drug delivery. To further understand the mechanisms involved in chitosan action on the mucosal Chitosan has been proposed as a novel excipient for transepithelial drug-delivery systems. Chitosan is thought to disrupt intercellular tight junctions, thus increasing the permeability of an epithelium. The effect of chitosan on tight junction complex was investigated at the molecular level. Methods. Changes in barrier properties of Caco-2 Although, the mucus layer was also reported to play a role in the access of chitosan to the epithelial membrane and the subsequent effect on the paracellular permeation route . Therefore, the effect of chitosan causing a change in permeation of acyclovir was tested in four different permeability models with and without a mucus layer. May 13, 2015Chitosan encapsulation modulates the effect of capsaicin on the tight junctions of MDCK cells V., Amin Khan, M. Merwin, J. R. Effect of chitosan on epithelial permeability and structure. Int Chitosan is thought to disrupt intercellular tight junctions, thus increasing the permeability of an epithelium. The effect of chitosan on tight junction complex was investigated at the molecular Jan 01, 2004Complex coacervates of DNA and chitosan could be used as a delivery vehicle in gene therapy and vaccine design[10-12], and have been shown to increase transcellular and paracellular transport of macromolecules across intestinal epithelial monolayers[13-15], further indicative of its potential in oral gene delivery.We compared the biophysical properties of free capsaicin and capsaicin-loaded chitosan nanocapsules, including their cytotoxicity towards epithelial MDCK-C7 cells and their effect on the buy cialis integrity of tight junctions, membrane permeability and cellular uptake.Chitosan encapsulation modulates the effect of capsaicin on the tight nanocarriers with enhanced epithelial permeability. TEM images of the nanocapsules revealed a core-shell structure E.K. Anderberg and P. Artursson. Epithelial transport of drugs in cell culture. VIII. The effects of the pharmaceutical surfactant excipient sodium dodecyl sulfate on cell membrane and tight junction permeability in human intestinal epithelial (Caco-2) cells.Abstract. Chitosan has been investigated as an excipient in the pharmaceutical industry, to be used in direct tablet compression, as a tablet disintegrant, for the production of controlled release solid dosage forms or for the improvement of drug dissolution.Repeated treatments with chitosan plus ciprofloxacin eliminate uropathogenic Escherichia coli reservoirs. Shown are bacterial titers in the bladders of mice that were infected with UTI89 and then left untreated or treated with 4 doses of chitosan alone or of chitosan plus ciprofloxacin.The aim of the present work is to investigate if conditions can be devised where PEGylation of chitosan would reduce its toxicity toward the nasal mucosa while maintaining its ability to open the cellular tight junctions and, consequently, produce an enhancement of macromolecular permeability. A series of mPEG-g-chitosan copolymers with varying levels of mPEG substitution, mPEG molecular Jan 01, 2013Chitosan (CS) is a potential paracellular permeation enhancer for trans-epithelial drug delivery; however, its ability to enhance epithelial permeability in a pH-dependent manner remains unclear. This study was designed to explore the underlying molecular mechanisms with regard to the effect of CS on tight junction (TJ) disruption at different A low-molecular weight chitosan (LCS) and a high-molecular weight chitosan (CS) were used as coating polymers of liposomes containing elcatonin (eCT). The muco-penetrative behaviors across the mucous gel layer covering the intestinal epithelial cells and the pharmacological effect after intragastric administration were determined in rats.The intestinal epithelial cells are mainly absorptive enterocytes (over 80%) but also include enteroendocrine, goblet, and Paneth cells . These epithelial cells are tightly bound together by intercellular junctional complexes that regulate the paracellular permeability and are crucial for the integrity of the epithelial barrier.This effect is likely to be mediated by loss of water from the thin fluid film covering the tracheal mucosa. This evaporation would give rise to formation of a hypertonic salt solution covering the mucosa, and, as shown in the present study, this could dramatically change the epithelial permeability by opening the tight junctions between the cells.Bacterial-derived lipopolysaccharides (LPS) can cause defective intestinal barrier function and play an important role in the development of inflammatory bowel disease. In this study, a nanocarrier based on chitosan and fucoidan was developed for oral delivery of berberine (Ber). A sulfonated fucoidan, fucoidan-taurine (FD-Tau) conjugate, was synthesized and characterized by Fourier transform Epithelial transport of drugs in cell culture. VII: Effects of pharmaceutical surfactant excipients and bile acids on transepithelial permeability in monolayers of human intestinal epithelial (Caco‐2) cellsIt was shown that the VA10 bronchial epithelial cell line was able to model the native bronchial epithelial integrity, permeability and morphology and can, therefore, be considered suitable to elucidate the relationship between the structure of N-quaternary chitosan derivatives and their activity as …Abstract Silibinin is a natural flavonoid with a strong antioxidant property and weak cytotoxic activity. It has demonstrated anti-tumoural activity against many types of malignancies; however, due to its hydrophobic structure, it has poor water solubility, bioavailability and permeability across intestinal epithelial … Oct 25, 2016Chitosan treated cells showed a thickened pattern of occludin at the cell periphery and not a disrupted one, which might be due to the ten-fold difference in concentration or to an effect exclusively related to the quaternized derivative of chitosan, TMC60.pharmaceutics Article Chitosan-Coated Nanoparticles: Effect of Chitosan Molecular Weight on Nasal Transmucosal Delivery Franciele Aline Bruinsmann 1,2, Stefania Pigana 2, Tanira Aguirre 3, Gabriele Dadalt Souto 1, Gabriela Garrastazu Pereira 1, Annalisa Bianchera 2, Laura Tiozzo Fasiolo 2,4, Gaia Colombo 4, Magno Marques 5, Adriana Raffin Pohlmann 1,6, Silvia Stanisuaski Guterres 1 and Article Permeability of Novel Chitosan-g-poly (Methyl Methacrylate) Amphiphilic Nanoparticles in a Model of Small Intestine in Vitro Imrit Noi 1,, Inbar Schlachet 1,, Muraly Kumarasamy 1 and Alejandro Sosnik 1,* 1 Laboratory of Pharmaceutical Nanomaterials Science, Department of …
